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8x sypro orange dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 8x sypro orange dye
    8x Sypro Orange Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sypro+orange+dye/Triton+X-100/pm42336208-485-35-39
    Average 99 stars, based on 1 article reviews
    8x sypro orange dye - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Supporting Information for Identification of potent pan-ephrin receptor kinase inhibitors using DNA- encoded chemistry technology
    Article Snippet: .. Denaturation of the EPHA2 and EPHA4 kinase domain proteins were monitored via an increase in fluorescence of SYPRO Orange dye (ThermoFisher Scientific, USA) which binds to hydrophobic residues that get exposed as the protein unfolds. ..

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: .. Briefly, Jun6504 was incubated at concentrations of 0, 3, 10, 30, 100, and 300 μM with 4 μM of either wild-type or mutant 2C protein at 37 °C for 1 h. After incubation, SYPRO Orange dye (Thermo Fisher) was added, and fluorescence was monitored under a temperature gradient from 30 to 90 °C, increasing at a rate of 0.05 °C per second. ..

    Article Title: Supporting Information for Network of epistatic interactions in an enzyme active site revealed by large-scale deep mutational scanning
    Article Snippet: .. Protein denaturation was monitored via an increase in fluorescence of SYPRO Orange dye (ThermoFisher Scientific, USA) which binds to hydrophobic residues that are exposed as the protein unfolds. ..

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: .. Briefly, Jun6504 was incubated at concentrations of 0, 3, 10, 30, 100, and 300 μM with 4 μM of either wildtype or mutant 2C protein at 37 °C for 1 h. After incubation, SYPRO Orange dye (Thermo Fisher) was added, and fluorescence was monitored under a temperature gradient from 30 to 90 °C, increasing at a rate of 0.05 °C per second. ..

    Incubation:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: .. Briefly, Jun6504 was incubated at concentrations of 0, 3, 10, 30, 100, and 300 μM with 4 μM of either wild-type or mutant 2C protein at 37 °C for 1 h. After incubation, SYPRO Orange dye (Thermo Fisher) was added, and fluorescence was monitored under a temperature gradient from 30 to 90 °C, increasing at a rate of 0.05 °C per second. ..

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: .. Briefly, Jun6504 was incubated at concentrations of 0, 3, 10, 30, 100, and 300 μM with 4 μM of either wildtype or mutant 2C protein at 37 °C for 1 h. After incubation, SYPRO Orange dye (Thermo Fisher) was added, and fluorescence was monitored under a temperature gradient from 30 to 90 °C, increasing at a rate of 0.05 °C per second. ..

    Mutagenesis:

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis
    Article Snippet: .. Briefly, Jun6504 was incubated at concentrations of 0, 3, 10, 30, 100, and 300 μM with 4 μM of either wild-type or mutant 2C protein at 37 °C for 1 h. After incubation, SYPRO Orange dye (Thermo Fisher) was added, and fluorescence was monitored under a temperature gradient from 30 to 90 °C, increasing at a rate of 0.05 °C per second. ..

    Article Title: A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.
    Article Snippet: .. Briefly, Jun6504 was incubated at concentrations of 0, 3, 10, 30, 100, and 300 μM with 4 μM of either wildtype or mutant 2C protein at 37 °C for 1 h. After incubation, SYPRO Orange dye (Thermo Fisher) was added, and fluorescence was monitored under a temperature gradient from 30 to 90 °C, increasing at a rate of 0.05 °C per second. ..

    Polymerase Chain Reaction:

    Article Title: FABP4 Couples Lipid Metabolism to PD-L1 Stabilization in Immunosuppressive Macrophages
    Article Snippet: .. Reactions were prepared in PCR tubes with a total volume of 20 μl, containing 10 μM FABP4 and 10× SYPRO Orange dye (Invitrogen) in buffer (20 mM HEPES, pH 7.0, 150 mM NaCl), along with either test compounds or ethanol controls. .. Samples were sealed, briefly centrifuged, and subjected to a temperature gradient from 25°C to 95°C at a rate of 1°C per minute using a 7500 Real-Time PCR system (Applied Biosystems).

    Labeling:

    Article Title: PPM1B utilizes a trinuclear metal architecture for phosphatase activity
    Article Snippet: .. PPM1B D151E was labeled with SYPRO Orange dye (ThermoFisher Scientific) and subjected to thermal denaturation using a Bio-Rad CFX Connect qPCR instrument in assay buffer (pH 7.0, 30 mM HEPES, 1 mM sodium ascorbate, 5 mM MnCl 2 , 0.01% Triton X-100, 5 μM PPM1B D151E protein) in the presence or absence of the phosphopeptide IKKβ(pS) (100 μM). ..

    Real-time Polymerase Chain Reaction:

    Article Title: PPM1B utilizes a trinuclear metal architecture for phosphatase activity
    Article Snippet: .. PPM1B D151E was labeled with SYPRO Orange dye (ThermoFisher Scientific) and subjected to thermal denaturation using a Bio-Rad CFX Connect qPCR instrument in assay buffer (pH 7.0, 30 mM HEPES, 1 mM sodium ascorbate, 5 mM MnCl 2 , 0.01% Triton X-100, 5 μM PPM1B D151E protein) in the presence or absence of the phosphopeptide IKKβ(pS) (100 μM). ..

    Phospho-proteomics:

    Article Title: PPM1B utilizes a trinuclear metal architecture for phosphatase activity
    Article Snippet: .. PPM1B D151E was labeled with SYPRO Orange dye (ThermoFisher Scientific) and subjected to thermal denaturation using a Bio-Rad CFX Connect qPCR instrument in assay buffer (pH 7.0, 30 mM HEPES, 1 mM sodium ascorbate, 5 mM MnCl 2 , 0.01% Triton X-100, 5 μM PPM1B D151E protein) in the presence or absence of the phosphopeptide IKKβ(pS) (100 μM). ..



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    Image Search Results


    ArgD binds CphB in both LAH and PAT conditions. A , the relative levels of ArgD and CphB under PAT and LAH in WT and Δ cphB were determined by immunodetection. Whole-cell lysates were separated on SDS-PAGE and blotted onto a PVDF membrane, and the ArgD and CphB proteins were detected using specific antibodies. The Ponceau staining of the membrane is shown for the loading control. Repetitions of the experiment and their statistical analysis are presented in . B , coimmunopurification of f.ArgD with CphB was performed using the same amounts of PAT- or LAH-grown f.argD/ Δ argD cells. The eluates were separated by SDS-PAGE together with the input lysates, including 50% of the lysate from the PAT-grown cells (PAT 50 ). The SYPRO-stained gel was subsequently blotted to a PVDF membrane, which was probed with specific antibodies against CphB and the FLAG tag of ArgD. The control f.ArgD pull-down prepared from the Δ cphB cells is shown in Ref. . CphB, cyanophycinase; f.ArgD, FLAG-tagged variant of ArgD; LAH, light-activated heterotrophic; PAT, photoautotroph; PVDF, polyvinylidene fluoride.

    Journal: The Journal of Biological Chemistry

    Article Title: Cyanophycinase is required for heterotrophy in cyanobacteria

    doi: 10.1016/j.jbc.2025.110791

    Figure Lengend Snippet: ArgD binds CphB in both LAH and PAT conditions. A , the relative levels of ArgD and CphB under PAT and LAH in WT and Δ cphB were determined by immunodetection. Whole-cell lysates were separated on SDS-PAGE and blotted onto a PVDF membrane, and the ArgD and CphB proteins were detected using specific antibodies. The Ponceau staining of the membrane is shown for the loading control. Repetitions of the experiment and their statistical analysis are presented in . B , coimmunopurification of f.ArgD with CphB was performed using the same amounts of PAT- or LAH-grown f.argD/ Δ argD cells. The eluates were separated by SDS-PAGE together with the input lysates, including 50% of the lysate from the PAT-grown cells (PAT 50 ). The SYPRO-stained gel was subsequently blotted to a PVDF membrane, which was probed with specific antibodies against CphB and the FLAG tag of ArgD. The control f.ArgD pull-down prepared from the Δ cphB cells is shown in Ref. . CphB, cyanophycinase; f.ArgD, FLAG-tagged variant of ArgD; LAH, light-activated heterotrophic; PAT, photoautotroph; PVDF, polyvinylidene fluoride.

    Article Snippet: The proteins were separated, visualized with SYPRO orange protein dye (Lumiprobe ProteOrange, catalog no.: 40210) and transferred onto a polyvinylidene fluoride membrane (Sigma–Aldrich, Immobilon-P, catalog no.: IPVH00010) that was subsequently incubated with primary anti-CphB and anti-ArgD antibodies ( ).

    Techniques: Immunodetection, SDS Page, Membrane, Staining, Control, FLAG-tag, Variant Assay